The classical BioBrick standard allows the free combination of most parts, but does not work for translational fusions, e.g. for addition of gfp or epitope-tags to protein-coding sequences. For this purpose, a number of BioBrick-compatible adaptations were developed, as described in RFC 23 and RFC 25 [11, 12]. In each case, the combination of parts is performed via standard restriction digests and subsequent ligations, preferably with vector backbones of different antibiotic resistances to allow the so-called 3A-assembly .
Acute chest pain syndromes algorithm using multi-parametric tissue characterisation. ECV denotes extra-cellular volume, LGE Late Gadolinium Enhancement, and MVO microvascular obstruction. . *This holds true for classical type 1 Takotsubo Cardiomyopathy 2b1af7f3a8